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A novel LC‐based method for the determination of enantiomeric excess (ee) in a sample mixture has been developed by employing on‐line 2D LC. The orthogonal separation system is composed of an NH2 column as the first dimension to elute the target chiral compound from the crude mixture and a chiral column as the second dimension to determine the ee of the target chiral product. A series of crude mixtures...
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