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A simple, reproducible and accurate RP-LC method has been developed for simultaneous analysis of seven flavonoids (quercimeritrin, hyperoside, isoquercetrin, astragalin, quercetin, quercetin-3′-glucoside, and tiliroside) in Flos Gossypii (FG), a drug used in Xinjiang, China. The analytes were separated on a C18 column by elution with a gradient prepared from methanol, acetonitrile, and 0.2% aqueous...
Enol and keto tautomers of methyl 3-oxo pentanoate could be separated on a HP-5 capillary column. The chromatographic peaks were identified by examining characteristic mass ions arose from the corresponding enol and keto molecular ions. The study showed that the area percentage of enol tautomer is a function of temperature of the column. Treating the column as a reactor, the energy of activation for...
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