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Here, we first present unique short (US)3, US4, and US5 gene sequences, with analysis, of duck enteritis virus (DEV) vaccine strain C-KCE. The assembled sequence comprises 5,742 nucleotides, which are amplified from the DEV genome by single oligonucleotide-nested polymerase chain reaction with primers designed according to our previous acquired sequence deposited in GenBank (accession no. EF619046)...
A 4554-bp fragment was amplified from the DEV C-KCE vaccine strain by single oligonucleotide nested polymerase chain reaction with partially known sequences for the DEV US1 and US10 genes. Three open reading frames containing the genes encoding US10, S3, and US2 were predicted using the Editseq program (DNAStar). The S3 and US2 genes have the same transcription orientation but are oriented head-to-head...
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