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Investigations using electrical impedance spectroscopy to measure the responses of fish embryos to the cryoprotective chemicals, methanol and dimethyl sulphoxide (DMSO), were carried out. Zebrafish (Danio rerio) embryos were used as a model to study the newly proposed technique. The normalised permittivity and conductivity changes of the embryos were measured continuously over a 20-min period in a...
The effect of partial removal of yolk on the survival of zebrafish embryos and the chilling sensitivity of yolk-reduced embryos were investigated at several stages of embryo development. Dechorionated embryos were punctured with a sharp microneedle and approximately 50 to 75% of yolk content was released following multiple punctures. The survival of yolk-reduced embryos was found to be stage dependent...
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