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In order to fully explore and comprehensive utilization the resource of Dictyoploca japonica, the pupa protein was extracted form pupa of Dictyoploca japonica by sodium hydroxide in this study. The pH of the solubility of protein had been determined. The best condition of extracting pupa protein: the concentration of NaOH is 1.5%, with the temperature 70??, the time 3 h and the pupa powder should...
It's well known that irreversible cross-linked complexes between proteins, produced by formaldehyde interacting with free amino or amide groups to form Schiff s base, are formed in diabetes and aging patients. To identify such specific modified peptides as biomarkers for clinical diagnose of diabetes and AD, cross-linkage of formaldehyde with human serum albumin (HSA) was analyzed by LC/ESI/MS in...
Aligning protein sequences using a score matrix has became a routine but valuable method in modern biological research. However, alignment in "Twilight Zone" is still a challenging issue. New score matrices such as Position Specific Score Matrix and structural context-based matrix have been issued to deal with it, whereas generalized matrices are still useful in pairwise alignment and/or...
Sequence alignment has been a most commonly used method in modern biological research. However, sequence alignment in "Twilight Zone" is still a challenging issue. Three protein class-specific matrices are constructed to investigate whether alignment in "Twilight Zone" can be improved by using class-specific matrices and gap penalties. The substitution pattern differences between...
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