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Pulsed electric fields (PEF) induced cellular damage in Saccharomyces cerevisiae, mainly focusing on metabolic properties including performance of mitochondria and cellular enzymes, was evaluated in this study. The proportion of sublethally injured S. cerevisiae cells was around 90% after PEF treatment at 20 kV/cm for 100–500 μs. Sublethal injure S. cerevisiae correlated with damage to mitochondria...
In this study, pulsed electric fields (PEF) treatments at 20 kV/cm for 0–500 μs were applied to Saccharomyces cerevisiae to investigate the effects of PEF on the cytomembrane and intracellular nucleic acids. The change in composition of membrane lipids characterized by decrease in the ratio of contents of unsaturated fatty acids versus saturated fatty acids induced by PEF was observed, which was related...
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