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The goal of this study was to create a novel baculovirus expression system that does not require recombinant virus purification steps. Transfection of insect cells with transfer vectors containing barnase under control of the Cotesia plutellae bracovirus (CpBV) promoters ORF3004 or ORF3005 reduced cell growth. Co-transfection with bApGOZA DNA yielded no recombinant viruses and non-recombinant backgrounds...
To investigate the usefulness of early-expressed promoters from Cotesia plutellae bracovirus (CpBV) in improvement of insecticidal activities of baculovirus, recombinant AcNPVs, Ac3003ProAaIT, Ac3004ProAaIT, Ac3005ProAaIT and Ac3006ProAaIT expressing scorpion neurotoxin, AaIT, under the control of ORF3003, ORF3004, ORF3005 and ORF3006 promoter, respectively were constructed. Among these recombinant...
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