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The expression and isolation of herpes simplex virus 1 (HSV-1) immediate early (α) IE63 (ICP27) and of the early (β) thymidine kinase (Tk) polypeptides in Escherichia coli JM 109 cells transformed with the PinPoint Xa-1 (Promega) plasmid construct carrying either the HSV-1 UL54 or UL23 genes are described. The resulting biotinylated fusion protein(s) could be easily induced and were purified in appropriate...
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