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Guanylate kinase is one of the key enzymes in nucleotide biosynthesis. The study highlights the structural and functional properties of Brugia malayi Guanylate kinase (BmGK) in the presence of chemical denaturants. An inactive, partially unfolded, dimeric intermediate was observed at 1–2M urea while GdnCl unfolding showed monomer molten globule like intermediate at 0.8–1.0M. The results also illustrate...
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