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A novel homogeneous ECL biosensing method for the determination of thrombin was designed based on DNA hybridization. As a model system, thrombin-binding aptamer was utilized as molecular recognition element and thrombin as a target analyte and ABEI derivatives as aptamer ECL label. In the absence of thrombin, thrombin-binding aptamer-tagged with ABEI produced a weak ECL signal. In the presence of...
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