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A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting the nucleocapsid phosphoprotein gene of infectious bronchitis virus (IBV) was developed. The detection limits for the IBV RT-LAMP assay were 10 1 50% egg infection dose (EID 50 ) per 50 μl of titrated viruses and no cross-reaction of IBV RT-LAMP was found when tested with other viruses including...
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