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Brown‐marbled grouper (Epinephelus fuscoguttatus) is a large‐bodied coral reef fish with high ecological and economic value in South‐East Asia. Previous studies have developed cryopreservation protocols in 0.25‐ or 0.5‐ml straws, but this small volume may limit the utilization of cryopreserved sperm for large‐scale artificial fertilization, considering the high fecundity of brown‐marbled grouper....
Production of cryopreserved semen in fish generally requires liquid nitrogen (LN), which is not always easily available in remote areas. To reduce reliance on LN, the aim of the present study was to evaluate whether electric freezer could be a feasible LN‐free alternative to cryopreserve brown‐marbled grouper sperm. After loading, semen straws were put directly into freezers (−30 or −80°C) for freezing...
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