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Omega–3 and omega–6 polyunsaturated free fatty acids (PUFA–FFA) are precursors to potent downstream lipid mediators that are regulators of inflammation. We describe the development and validation of a novel and sensitive method for quantification of individual PUFA–FFA in a dried blood spot using liquid chromatography tandem mass spectrometry (LC–MS/MS). Lipids were extracted from dried blood spot...
Conventional assays of omega-3 long chain polyunsaturated fatty acid (n-3 LCPUFA) status in humans involve venous blood collection and expensive, multi-step processes that limit their usefulness as screening tools. This study aimed to develop a capillary dried blood spot (DBS) system capable of protecting n-3 LCPUFA from oxidation for up to 2 months at room temperature (20–25°C). We demonstrated that...
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