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The glnA gene encoding glutamine synthetase (GS) was amplified from Azospirillum brasilense Sp7 with PCR technique. The amplified 1.4-kb DNA fragment flanked with a BamH I site at each end was cloned into EcoR V site of Bluescript-SK vector. A recombinant plasmid pGSJ1 containing this 1.4-kb DNA fragment was selected by restriction digestion analysis. The sequencing data also confirmed that the...
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