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One of the main problems of the nanoparticle dispersion state change (e.g. from dispersion to aggregation) based surface-enhanced Raman scattering (SERS) detection is that the dynamic process of such in-solution reactions is always uncontrollable. This leads to poor reproducibility from a narrow time window of all such strategies, and finally great difference between the data from the diverse methods,...
Because amyloidogenic proteins, such as prion protein, β-amyloid peptide and α-synuclein, are associated with a variety of diseases, methods for their detection are important. Recombinant prion protein (rPrP) can selectively induce aggregation of dihydrolipoic acid capped gold nanoparticles (DHLA-AuNPs), which reduces the absorbance of the DHLA-AuNPs and changes their color from red to blue. These...
CdTe quantum dots (QDs) were used as a highly selective probe for the detection of prion protein. Orange-emitting precipitates appeared within 30s of the addition of recombination prion protein (rPrP) to a solution of green-emitting CdTe QDs. This allowed colorimetric qualitative and semi-quantitative detection of rPrP. The decrease in fluorescence intensity of the supernatant could be used for quantitative...
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