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Many studies have established microRNAs (miRNAs) as post-transcriptional regulators in a variety of intracellular molecular processes. Abnormal changes in miRNA have been associated with several diseases. However, these changes are sometimes subtle and occur at nanomolar levels or lower. Several biosensing hurdles for in situ cellular/tissue analysis of miRNA limit detection of small amounts of miRNA...
A real-time fluorescence detection biomedical system with a microfluidic chip was developed for loop-mediated isothermal amplification (LAMP) reactions. Two kinds of microfluidic chips with linear microchannels or microcells were designed and fabricated by MEMS technology and thin-casting method. Both traditional centrifugal tubes and microfluidic chips were used for LAMP reactions. Based on the experimental...
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