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Ribosomal protein S3A (RPS3A) is a component of the 40s small ribosomal subunit, which plays an important role in ribosome. To explore the structure characteristic of RPS3A gene of giant panda (Ailuropoda Melanoleuca), the cDNA and the genomic sequence of RPS 3A were cloned successfully from the giant panda by RT-PCR and Touch-down technology respectively, followed by sequencing and analysing. The...
Ribosomal protein L10A is a component of the 60S large ribosomal subunit. To explore the structure feature of RPL10A gene of the Ailuropoda melanoleuca,the cDNA and the genomic sequence of RPL10A gene were cloned using RT-PCR and touchdown -PCR technology. The obtained cDNA of RPL10A in giant panda is 689 bp in size, containing an ORF of 654 bp, and the deduced protein was composed of 217 amino acids...
To explore the structure characteristic of rpL14 gene of A. melanoleuca and the differences among other species reported, we have designed primers based on the known information of rpL14 gene. Then, the cDNA and the genomic sequence of rpL14 gene were cloned successfully from A. melanoleuca by RT-PCR and PCR strategy respectively, and followed by sequencing and analyzing. The result indicated that...
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