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•A simple, mild, one-pot tandem method catalyzed by trypsin was developed for the synthesis of 3,4-dihydropyrimidin-2(1H)-ones.•Trypsin was found to display dual promiscuous functions to catalyze transesterification and the Biginelli reaction in sequence.•A Biginelli reaction using in situ-produced acetaldehyde was developed.•The 3,4-dihydropyrimidin-2(1H)-ones were synthesized through one-pot tandem...
The tripeptide BzArgGlyAsp(NH 2 ) 2 was synthesized by a combination of chemical and enzymatic methods in this study. First of all, GlyAsp(NH 2 ) 2 was synthesized by a novel chemical method in three steps including chloroacetylation of l-aspartic acid, esterification of chloroacetyl l-aspartic acid and ammonolysis of chloroacetyl l-aspartic acid diethyl ester. Secondly,...
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