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A food-grade host/vector expression system for Lactococcuslactis was constructed using alanine racemase gene (alr) as the complementation marker. We obtained an alanine racemase auxotrophic mutant L. lactis NZ9000Δalr by double-crossover recombination using temperature-sensitive integration plasmid pG+host9 and a food-grade vector pALR with entirely lactococcal DNA elements, including lactococcal...
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