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A 3-kbp DNA fragment including the l-2,3-butanediol dehydrogenase (l-BDH) gene (budC) from the chromosomal DNA of Brevibacterium saccharolyticum C-1012 was cloned in Escherichia coli JM109 after its insertion into pBluescript II SK + , and the resulting plasmid was named pLBD-SK. The budC had an open reading frame consisting of 774 bp and encoded 258 amino acids. It was not included in a 2,3-butanediol...
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