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A sensitive and stable bioassay for the detection of Aβ oligomer (Aβo), a potentially promising candidate biomarker for Alzheimer's disease (AD) diagnosis, was developed using Fe3O4 magnetic nanoparticles (MNPs) as the recognition and concentration elements and BaYF5:Yb,Er upconversion nanoparticles (UCNPs) as highly sensitive labels, conjugated with the Aβo aptamer (DNA1) and the complementary oligonucleotide...
A novel water-soluble fluorescent probe FLS2 based on the thiooxofluorescein derivative has been firstly designed and synthesized. UV–vis absorption and fluorescence spectra studies showed that the FLS2 as a colorimetric and ratiometric fluorescent probe exhibited high selectivity and sensitivity towards Hg2+, which was mainly attributed to the special binding with the receptor unit accompanied with...
A hybrid carbazole-hemicyanine dye (Cac) has been developed as a novel colorimetric and ratiometric fluorescent sensor for cyanide detection. Upon treatment with cyanide, Cac displayed a remarkable fluorescence ratiometric response, with the emission wavelength displaying a very large emission shift (214nm). The detection of cyanide was performed via the nucleophilic addition of cyanide anion to the...
With the biomolecule glutathione (GSH) as a capping ligand, Eu 3+ -doped cadmium sulfide composite nanoparticles were successfully synthesized through a straightforward one-pot process. An efficient fluorescence energy transfer system with CdS nanoparticles as energy donor and Eu 3+ ions as energy accepter was developed. As a result of specific interaction, the fluorescence intensity...
The interactions of brilliant cresyl violet (BCV) with herring sperm DNA in γ-cyclodextrin (γ-CD) supramolecular system were studied by UV-vis absorption spectroscopy and cyclic voltammetry (CV). Both UV-vis absorption and CV data show that the interaction of BCV with DNA depends on the concentration ratio of BCV to DNA (R), the initial concentration of BCV and γ-CD. The binding constants of BCV monomer,...
A new method of electrochemical probe has been proposed for the determination of Herring Sperm DNA (DNA) based on its interaction with Basic Brown G (BBG). The electrochemical behavior of interaction of BBG with DNA was investigated on Hg electrode. In 0.1molL −1 NH 3 –NH 4 Cl buffer solution (pH 8.0), BBG can be reduced on Hg electrode with a well-defined voltammetric peak...
Mesoporous materials were employed as fast, sensitive and efficient fiber coatings of solid-phase microextraction (SPME) for the first time. Three micrometer as-synthesized C 16 -MCM-41 particles were immobilized onto stainless steel wire with 100μm coating thickness. In combination with high performance liquid chromatography (HPLC), extraction efficiency and selectivity of C 1 ...
A liquid ion-selective membrane electrode containing a chloroform-acetonitrile (10:1) solution of the complex of In(III) with 1-benzyl-3-methyl-4-benzoyl-5-pyrazolone (PMBP) and diphenylguanidine is described. The basic electrode performance characteristics were evaluated according to IUPAC recommendations. The slope of the calibration graph (electrode potential v. concentration) was 18.7 mV (pIn)...
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