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Super resolution localization microscopy (SRLM) techniques such as STORM and PALM overcome the ∼200nm diffraction limit of conventional light microscopy by randomly activating separate fluorophores over time and computationally aggregating their nanometer resolution detected locations for image reconstruction. However, a basic limitation of current SRLM approaches for live cell imaging is their low...
Quantum dots (QDs) are semiconductor nanocrystals whose unique fluorescence properties make them desirable biological imaging probes. However, reliable and efficient cellular delivery of QDs remains technically challenging. To address this problem, we developed a cell penetrating peptide (CPP) based approach that delivers QDs into mammalian cells with high reproducibility and efficiency and minimal...
We propose an automated algorithm for segmentation of mitochondria from widefield fluorescence microscopy images for quantitative morphology characterization. Mitochondria are membrane-bound organelles that are essential to cells of higher living organisms. Reliable and precise quantitative characterization of their shape is crucial to understanding related physiology and disease mechanisms. Building...
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