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This study was conducted to build a recombinant strain with highly insecticidal activity and a wide host range by using the cry 1 Ac and p74 gene. Firstly, the p74 gene was amplified from the genosome of Autographa californica multicapsid nucleopolyhedrovirus. The cry1Ac gene and the terminator gene of cry1Ac, named cry1Act, were amplified from the plasmid of Bt 4.0718 strain. Three T vectors, named...
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