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We have investigated the changes in insulin-like growth factor 1 (IGF-1), IGF binding protein (IGFBP)-3, and sex steroids in normal male and female rats. Blood samples were obtained from 3-, 5-, 7-, 9-, 12-, 15-, and 20-week-old Wistar-Imamichi rats. In males, marked increases in serum IGF-1 and IGFBP-3 levels appeared earlier than that in serum testosterone level. IGF-1 and testosterone peaked...
We have examined the expression of both pituitary adenylate cyclase-activating polypeptide (PACAP) and PACAP receptor gene in the rat ovary using RT-PCR. mRNA was prepared from pregnant mare serum gonadotropin (PMSG)-treated, PMS-human chorionic gonadotropin (hCG)-treated, and hormone-untreated immature rat ovaries. mRNA from each group yielded a dominant DNA band corresponding in size to the PACAP...
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