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NEMO is an essential regulatory component of the IκB kinase (IKK) complex, which controls activation of the NF-κB signaling pathway. Herein, we show that NEMO exists as a disulfide-bonded dimer when isolated from several cell types and analyzed by SDS–polyacrylamide gel electrophoresis under non-reducing conditions. Treatment of cells with hydrogen peroxide (H 2 O 2 ) induces further...
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