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Flavin mononucleotide (FMN) is a highly efficient photosensitizer (PS) yielding singlet oxygen (1O2). However, its 1O2 production efficiency significantly decreases upon isoalloxazine ring encapsulation into the protein matrix in genetically encoded photosensitizers (GEPS). Reducing isoalloxazine ring interactions with surrounding amino acids by protein engineering may increase 1O2 production efficiency...