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The efficacy of candidate DNA vaccines based on the smallpox natural gene A30L and artificial gene A30Lopt with modified codon composition optimized for expression in mammalian cells was tested. Groups of mice were intracutaneously immunized three times at 3-week intervals with candidate DNA vaccines, i.e., pcDNA_A30L or pcDNA_A30Lopt. Three weeks after the last immunization, all groups of mice were...
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