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LC‐MS/MS is the most commonly used technique for the identification and characterization of proteins. The efficiency of the electrospray process is a critical factor in LC‐MS/MS. Despite the benefits associated with very low flow rates for the ionization efficiency, most LC‐MS/MS platforms are operated at relatively high flow rates. The purpose of this work was to develop a nano LC system operable...
In the last 15 years, MS‐based protein characterization has expanded at a rapid rate. This success is built upon constantly improving instrumentation and a variety of ingenious methods applied to numerous biological questions. However, the reproducibility of mass spectrometric results is considered by many as insufficient. In part, inadequate quality control might be responsible for the lack of reproducibility...
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