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The development of nematode feeding sites induced by root‐knot nematodes involves the synchronized activation of cell cycle processes such as acytokinetic mitoses and DNA amplification. A number of key cell cycle genes are reported to be critical for nematode feeding site development. However, it remains unknown whether plant cyclin‐dependent kinase (CDK) inhibitors such as the Arabidopsis interactor/inhibitor...
•Excellent visualization of nuclei was obtained here using a whole‐mount procedure adapted to provide high‐resolution images of large, irregularly shaped nuclei. The procedure is based on tissue clearing, and fluorescent staining of nuclear DNA with the dye propidium iodide.
•The method developed for standard confocal imaging was applied to large multicellular root swellings, named galls, induced...
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