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The growth of the truffle, Tuber borchii, mycelial strain (1BO) was analysed using biomolecular methods. Total RNA evaluation and ergosterol assay revealed that transcriptional and metabolic activity reach a peak at around 30–50 days of growth and decrease by 70 days. mRNA electrophoretic profiles highlighted a very low degree of variation in gene expression during the growth of mycelium.
The mRNA differential display technique was used to compare mRNA populations from fruit body and mycelium of a white truffle species in the attempt to identify and clone differentially expressed genes. The differential expression of five out of 30 amplicons was confirmed. One fragment (Tbm 56) corresponded to a part of the ribosomal genes. Three cDNA fragments (Tbf 12, Tbf 20, Tbf 21) were expressed...
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