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To reveal the intracellular localization of Hsp104 in the yeastSaccharomyces cerevisiaebefore and after heat-shock, we performed immunoelectron microscopy after immunogold labeling with anti-Hsp104 antibody. At normal temperature (25°C), a small amount of Hsp104 was located in the cytoplasm and nucleus. On exposure to mild heat-shock at 40°C, protein aggregates appeared in the cytoplasm and nucleus,...
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