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A cold-passage (cp) temperature-sensitive (ts) RSV mutant, designated RSV cpts-530, which possesses host-range mutations acquired during 52 passages at low temperature in bovine tissue culture and one or more ts mutations induced by chemical mutagenesis (shut-off temperature 39°C) was found previously to be tenfold restricted in its replication in mice as compared to wild-type virus and stable genetically...
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