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The primers and probes were designed and synthesized according to the conserved VP1 sequences of porcine Sapovirus (SaV), and a TaqMan fluorescence quantitative RT-PCR assay were developed by optimizing the reaction conditions. Results showed that the fluorescence quantitative RT-PCR assay could detect 16.1 copies·µL−1 of plasmid DNA, while the sensitivity of the routine RT-PCR was 1.61 × 103 copies·µL...
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