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A highly simplified and rapid enzymatic procedure for the determination of lactulose was developed. Lactulose was hydrolysed to fructose and galactose by β-galactosidase, and the amount of fructose was determined by using immobilized fructose dehydrogenase and K 3 [Fe(CN) 6 ] as mediator. The reduced mediator was re-oxidised at a screen-printed Pt-electrode at a potential of 385 mV...
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