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A thermophilic bacterium was isolated from a hot spring area of Yellowstone National Park. The organism grew optimally at 60-65 o C and in the pH range of 6-9. It was characterized as Bacillus sp. In the presence of corn or olive oil (1.0%) as the growth substrate, this Bacillus produced an extracellular lipolytic activity (EC 3.1.1.3). The enzyme activity could be efficiently recovered by...
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