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We report a simple glycol methacrylate (GMA) cold embedding technique that can be used on bone marrow biopsy core. The biopsies were fixed in cold Bouins solution, dehydrated in cold methanol followed by infiltration and embedding in cold GMA then polymerised at 4C under vacuum. This provided an effective cooling system, which preserved a wide range of the cell antigens by preventing heat damage due...
We report a simple glycol methacrylate (GMA) cold embedding technique that can be used on bone marrow biopsy core. The biopsies were fixed in cold Bouin’s solution, dehydrated in cold methanol followed by infiltration and embedding in cold GMA then polymerised at 4°C under vacuum. This provided an effective cooling system, which preserved a wide range of the cell antigens by preventing heat damage...
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