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At present, the accuracy of clinical hepatocellular carcinoma (HCC) diagnosis needs to be further improved. In this work, two kinds of serological biomarker species, microRNA and protein biomarker, have been detected simultaneously to identify HCC. Herein, a dual-aptamer hairpin DNA oligonucleotide is designed as the electrochemical sensing probe (ESP) to achieve this goal. The hairpin-structured...
Trinucleotide repeats (TNRs) are involved in a number of debilitating diseases and disorders, the length of which usually indicates disease severity at gene level. Herein we have developed a novel fluorescent method in this work to evaluate TNRs length polymorphism based on its DNA secondary structure with doxorubicin (Dox) as fluorescent probe. This new method makes use of the fact that TNRs rich...
Non-negative matrix factorization (NMF), with the constraints of non-negativity, has been recently proposed for multi-variate data analysis. Because it allows only additive, not subtractive, combinations of the original data, NMF is capable of producing region or parts-based representation of objects. It has been used for image analysis and text processing. Unlike PCA, the resolutions of NMF are non-negative...
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