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Stable isotope probing of prokaryotic DNA was used to determine active prokaryotes using 13C‐labelled substrates (glucose, acetate, CO2) in sediment slurries from different biogeochemical zones of the Severn Estuary, UK. Multiple, low concentrations (5 × 100 μM) of 13C‐substrate additions and short‐term incubations (7 days) were used to minimize changes in the prokaryotic community, while achieving...
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