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A radiation inactivation technique was employed to determine the functional size of pyrophosphatase (PPase) from the chromatophores of Rhodospirillum rubrum. The activities of hydrolysis and synthesis reactions of pyrophosphatase and its coupled proton translocation decayed in a simple exponential function with the increase of radiation dosages. D 3 , values of 5.2 +/- 0.7 and 5.8 +/- 0.8...
The technique of (γ-ray) radiation inactivation has been used to estimate the functional sizes of the PS II centers (PS II α and PS II β ) responsible for the biphasicity of the fluorescence induction curve from DCMU-inhibited spinach chloroplasts. It is found that the size of PS II α (266 +/- 5 kDa) is almost twice as large as that of PS II β (135 +/- 5 kDa). The result...
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