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Due to drawbacks of live attenuated vaccines, much attention has been focused on screening Brucella‐protective antigens as subunit vaccine candidates. Here, an immunoproteomic assay was used to identify the immunogenic soluble proteins of Brucella melitensis 16M. In the present study, 27 unique immunogenic proteins were identified from the two‐dimensional electrophoresis immunoblot profiles by liquid...
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