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We generated and characterized a convenient isolated cell model of human detrusor smooth muscle to understand mechanisms that may underlie detrusor instability and provide a suitable model to test potentially useful drugs. The electrophysiological properties of freshly isolated detrusor smooth muscle cells from human and guinea pig biopsies were compared with those undergoing cell culture to document...
We carefully examined the possible routes of Ca 2+ influx, and determined whether cultured cells retain Ca 2+ channels and whether the culturing process changes their properties. Inward currents were measured under voltage clamp in freshly isolated cells and myocytes from confluent cell cultures of detrusor smooth muscle. In guinea pig and human cells mean peak inward current...
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