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In order to investigate the effects of electric pulses on cancer cells, HepG2 and L02 were exposed to electric pulses (1000V/cm, 100μs, 1Hz) for different time (8s, 15s, 30s, 60s). Annexin-V-FITC Kit and Flow cytometry were used to study the apoptosis of treated cells. The results showed that the electric pulses of 1000V/cm, 100μs, 1Hz for 8s couldn't induce tumor cells apoptosis. Apoptosis was observed...
To research the apoptosis effects of steep pulsed electric fields (SPEF) on human liver cancer cell SMMC-7721 in vitro, this paper studied phosphatidylserine (PS) externalization detection utilizing flow cytometry, real-time change of mitochondrial transmembrane potential (DeltaPsim) using confocal laser scanning microscope (CLSM) and DNA fragmentation assay by agarose gel electrophoresis. The experiment...
Mitochondrial plays an important role in apoptosis, and measuring the change of mitochondrial transmembrane potential (DeltaPsim) is a useful method for apoptosis. Exposing human liver cancer cell SMMC-7721 dyed with Rhodamine 123 to steep pulsed electric fields (SPEF), this paper observed the real-time change of DeltaPsim using laser scanning confocal microscope (LSCM) to study the apoptosis effect...
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