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The enzyme phospholipase Cγ1 (PLCγ1) is essential for T cell signaling and activation. Following T cell receptor ligation, PLCγ1 interacts through its SH2 and SH3 domains with the adaptors LAT and SLP-76, respectively, to form a multiprotein signaling complex that leads to activation of PLCγ1 by Syk tyrosine kinases. To identify the binding site for PLCγ1 in SLP-76, we used isothermal titration calorimetry...
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