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Objective To identify potential tumor antigen by immunoscreening the urinary bladder cancer cDNA library with monoclonal antibodies. Methods Monoclonal antibodies were prepared. A cDNA expression library was constructed from bladder cancer cell line BLZ211. Immunogenic proteins were identified by immunoscreening the cDNA library with ten monoclonal antibodies. Results The cDNA library of BLZ211...
A non-protein-coding RNA, UCA1, has been cloned from human bladder TCC cell line BLZ-211 by using 5′ and 3′ RACE. The UCA1 full-length cDNA was 1442 bp. RT-PCR analysis indicated that UCA1 is an embryonic development and bladder cancer-associated RNA. The proliferative, migrative, invasive, and drug resistance behaviors of human bladder TCC cell line BLS-211 were enhanced by exogenous UCA1 expression...
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