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To develop a practical procedure for cryopreservation of adult bovine testis tissue, the impacts of three cryoprotectans and their concentrations, as well as varying thawing temperatures, on the cell viability of bovine testis tissue after freezing/thawing were examined. The highest testicular cell viabilities came from the media containing dimethyl sulphoxide (DMSO, 85.3±1.2%), propylene glycol (PG,...
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