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A novel surface plasmon resonance (SPR) biosensor for the determination of human IgG was introduced. The biosensor was prepared with three layers of titania sol–gel membrane by vapor deposition. The colloid Au nanoparticle (AuNP) was immobilized in the second layer of titania membrane and the AuNP coupled with rabbit anti-human IgG was encapsulated in the third layer. The AuNP in the second layer...
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