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Urokinase plasminogen activator receptor (uPAR) exists in a number of formats in human plasma, including soluble uPAR (suPAR) and uPAR fragments. We developed an ELISA method to detect specifically the active form suPAR, which binds to its natural ligand uPA. The intra CV and inter CV of this ELISA assay is 8.5% and 9.6% respectively, and the assay can recover 99.74% of added recombinant suPAR from...
Analysis of water and sand samples was done by reflectance measurements using a mobile phone. The phone's screen served as light source and front view camera as detector. Reflected intensities for white, red, green and blue colors were used to do principal component analysis for classification of several compounds and their concentrations in water. Analyses of colored solutions and colorimetric reactions...
A new, simple, economical and validated high-performance liquid chromatography linked with electrochemical detector (HPLC–ECD) method has been developed and optimized for different experimental parameters to analyze the most common monothiols and disulfide (cystine, cysteine, homocysteine, methionine, reduced (GSH) and oxidized glutathione (GSSG)) and ascorbic acid present in human plasma and erythrocytes...
Normal phase ion-pair high performance liquid chromatography has been used for simultaneous separation of nitrite and nitrate using tetraethylammonium (TEA) + as ion-pairing reagent. The concentration effect of (TEA) + , buffer salt and pH of the eluent on separation is investigated. The UV detector response at various wavelengths has been optimized. The performance of the proposed...
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