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An insect depressant toxin Buthus martensii Karsch insect toxin 4 (BmK IT 4 ) cDNA was cloned into the prokaryotic expression vector pSW202 and expressed in HB101 host cells. The authenticity of this in vitro expressed peptide was confirmed by Western blotting, mass spectrometry and N-terminal peptide sequencing. Bioassays using growth media supplemented with BmK IT 4 demonstrated...
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