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The discharge process in vacuum is usually classified into two types, micro particle type and field emission type. The influence of particles has been experimentally investigated using a high speed camera. However, few studies have reported the quantitative analysis on the particle type discharge. To understand the micro particle type discharge better, the collision phenomena between a sphere particle...
Intense nanoseconds pulsed electric field (nsPEF) is a novel physical stress to organisms, and has been reported to induce various unique responses to mammalian cells, such as bleb formation, phosphatidylserine externalization, increase in calcium ion concentration1, as well as increase in membrane permeabilization. The primary biological effect of nsPEF depends on the pulse parameters such as pulse...
Biological effects of intense pulsed electric fields (PEF) have been intensively investigated in the last decade. PEFs are c apable of giving biological targets unique stress dependent on its pulse duration, rise time and field strength. In general, nanosecond pulsed electric fields (nsPEF) cause intracellular effects, while micro-to-millisecond long pulses affect the cell membrane. We are using an...
Here, we demonstrates the influence of the pulsing sequence of repetitive nanosecond pulsed electric fields on the viability of human cancer cells. A commercially available repetitive magnetic pulse compression generator was used to deliver 70 ns-long high voltage pulses to cultured HeLa cells in a 4 mm-gap cuvette electrode. Two kinds of pulsing sequential patterns, simply repetitive ns pulses and...
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