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Acorus calamus L (sweet flag) is a semi-aquatic perennial medicinal herb having a creeping and much branched aromatic rhizome. Rhizome buds cultured in MS basal medium supplemented with BAP (8.87μM) and NAA (5.37μM) at light intensity of 30μE m−2 s−1 and photoperiod of 12 h produced 8–10 multiple shoots by the eighth week of inoculation. Shoots could be rooted in MS basal medium without growth regulators...
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